[Histonet] Need silver stain for granules in adrenals

[Geoff McAuliffe]

I don't think you can get a good chromaffin reaction on material already 
fixed with formalin. Fontana-Masson should be fine. John Kiernan 
published a chromate-dichromate fix for chromaffin tissues, it may be in 
his book, along with other recommendations.

Geoff

Nick Kirk wrote:

> What you are trying to demonstrate are Chromaffin cells of the adrenal 
> glands so a Masson-Fontana should work, if not try a Schmorl or carry 
> out the Chromaffin reaction
> If you use Iodate oxidation you can distinguish between Adrenaline and 
> noradrenalin fairly easily as iodates oxidise noradrenaline much 
> quicker than they do adrenaline.
>  
> Nick Kirk
> Histopathology
> Hinchingbrooke Hospital
> Huntingdon
> England
>
>     -----Original Message-----
>     From: histonet-admin <@t> lists.utsouthwestern.edu
>     [mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Nancy
>     Maronto
>     Sent: 09 October 2003 16:05
>     To: histonet <@t> lists.utsouthwestern.edu
>     Subject: [Histonet] Need silver stain for granules in adrenals
>
>     Hi,
>     Could anyone suggest a stain for noradrenalin and adrenalin
>     granules in paraffin embedded adrenals.  We were asked to do a
>     gremilius stain and it did not stain the granules.  Are these
>     slides lost or can we re stain them?  We usually do a recut of the
>     block, but it is asked if we could use the same tissue slide. The
>     stain actually worked, but not for the granules.
>      
>     From what we read a Fontana-masson should work.  There probably is
>     a better stain our there for this specific target.  Can anyone
>     with some experience with staining these granules share their
>     staining protocol.
>      
>     At this time the request is not for immuno staining. 
>      
>     Thanks for your help.
>      
>     Nancy Maronto
>

-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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