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I don't think you can get a good chromaffin reaction on material
already fixed with formalin. Fontana-Masson should be fine. John
Kiernan published a chromate-dichromate fix for chromaffin tissues, it
may be in his book, along with other recommendations.<br>
<br>
Geoff<br>
<br>
Nick Kirk wrote:<br>
<blockquote type="cite"
cite="midDHEJLPLDFDGJCMMAGCIGCEEMCBAA.nick.kirk3@btopenworld.com">
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<div><font face="Arial" color="#0000ff" size="2"><span
class="046044117-09102003">What you are trying to demonstrate are
Chromaffin cells of the adrenal glands so a Masson-Fontana should work,
if not try a Schmorl or carry out the Chromaffin reaction</span></font></div>
<div><font face="Arial" color="#0000ff" size="2"><span
class="046044117-09102003">If you use Iodate oxidation you can
distinguish between Adrenaline and noradrenalin fairly easily as
iodates oxidise noradrenaline much quicker than they do adrenaline.</span></font></div>
<div> </div>
<div><font face="Arial" color="#0000ff" size="2"><span
class="046044117-09102003">Nick Kirk</span></font></div>
<div><font face="Arial" color="#0000ff" size="2"><span
class="046044117-09102003">Histopathology</span></font></div>
<div><font face="Arial" color="#0000ff" size="2"><span
class="046044117-09102003">Hinchingbrooke Hospital</span></font></div>
<div><font face="Arial" color="#0000ff" size="2"><span
class="046044117-09102003">Huntingdon</span></font></div>
<div><font face="Arial" color="#0000ff" size="2"><span
class="046044117-09102003">England</span></font></div>
<blockquote dir="ltr" style="margin-right: 0px;">
<div class="OutlookMessageHeader" dir="ltr" align="left"><font
face="Tahoma" size="2">-----Original Message-----<br>
<b>From:</b> <a class="moz-txt-link-abbreviated" href="mailto:histonet-admin@lists.utsouthwestern.edu">histonet-admin@lists.utsouthwestern.edu</a>
[<a class="moz-txt-link-freetext" href="mailto:histonet-admin@lists.utsouthwestern.edu">mailto:histonet-admin@lists.utsouthwestern.edu</a>]<b>On Behalf Of </b>Nancy
Maronto<br>
<b>Sent:</b> 09 October 2003 16:05<br>
<b>To:</b> <a class="moz-txt-link-abbreviated" href="mailto:histonet@lists.utsouthwestern.edu">histonet@lists.utsouthwestern.edu</a><br>
<b>Subject:</b> [Histonet] Need silver stain for granules in
adrenals<br>
<br>
</font></div>
<div>Hi,</div>
<div>Could anyone suggest a stain for noradrenalin and adrenalin
granules in paraffin embedded adrenals. We were asked to do a
gremilius stain and it did not stain the granules. Are these slides
lost or can we re stain them? We usually do a recut of the block, but
it is asked if we could use the same tissue slide. The stain actually
worked, but not for the granules.</div>
<div> </div>
<div>From what we read a Fontana-masson should work. There
probably is a better stain our there for this specific target. Can
anyone with some experience with staining these granules share their
staining protocol.</div>
<div> </div>
<div>At this time the request is not for immuno staining. </div>
<div> </div>
<div>Thanks for your help.</div>
<div> </div>
<div>Nancy Maronto</div>
</blockquote>
</blockquote>
<br>
<pre class="moz-signature" cols="72">--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
<a class="moz-txt-link-abbreviated" href="mailto:mcauliff@umdnj.edu">mcauliff@umdnj.edu</a>
**********************************************</pre>
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