[Histonet] Need silver stain for granules in adrenals

[Nick Kirk]

What you are trying to demonstrate are Chromaffin cells of the adrenal
glands so a Masson-Fontana should work, if not try a Schmorl or carry out
the Chromaffin reaction
If you use Iodate oxidation you can distinguish between Adrenaline and
noradrenalin fairly easily as iodates oxidise noradrenaline much quicker
than they do adrenaline.

Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England
  -----Original Message-----
  From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Nancy Maronto
  Sent: 09 October 2003 16:05
  To: histonet <@t> lists.utsouthwestern.edu
  Subject: [Histonet] Need silver stain for granules in adrenals


  Hi,
  Could anyone suggest a stain for noradrenalin and adrenalin granules in
paraffin embedded adrenals.  We were asked to do a gremilius stain and it
did not stain the granules.  Are these slides lost or can we re stain them?
We usually do a recut of the block, but it is asked if we could use the same
tissue slide. The stain actually worked, but not for the granules.

  From what we read a Fontana-masson should work.  There probably is a
better stain our there for this specific target.  Can anyone with some
experience with staining these granules share their staining protocol.

  At this time the request is not for immuno staining.

  Thanks for your help.

  Nancy Maronto
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