[Histonet] Need silver stain for granules in adrenals

[Nick Kirk]

You could try secondary fixing the tissue in chromate solution to get the
Chromaffin reaction, it usually works.

Nick Kirk
  -----Original Message-----
  From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Geoff McAuliffe
  Sent: 09 October 2003 22:15
  To: Nick Kirk
  Cc: Histonet
  Subject: Re: [Histonet] Need silver stain for granules in adrenals


  I don't think you can get a good chromaffin reaction on material already
fixed with formalin. Fontana-Masson should be fine. John Kiernan published a
chromate-dichromate fix for chromaffin tissues, it may be in his book, along
with other recommendations.

  Geoff

  Nick Kirk wrote:

    What you are trying to demonstrate are Chromaffin cells of the adrenal
glands so a Masson-Fontana should work, if not try a Schmorl or carry out
the Chromaffin reaction
    If you use Iodate oxidation you can distinguish between Adrenaline and
noradrenalin fairly easily as iodates oxidise noradrenaline much quicker
than they do adrenaline.

    Nick Kirk
    Histopathology
    Hinchingbrooke Hospital
    Huntingdon
    England
      -----Original Message-----
      From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Nancy Maronto
      Sent: 09 October 2003 16:05
      To: histonet <@t> lists.utsouthwestern.edu
      Subject: [Histonet] Need silver stain for granules in adrenals


      Hi,
      Could anyone suggest a stain for noradrenalin and adrenalin granules
in paraffin embedded adrenals.  We were asked to do a gremilius stain and it
did not stain the granules.  Are these slides lost or can we re stain them?
We usually do a recut of the block, but it is asked if we could use the same
tissue slide. The stain actually worked, but not for the granules.

      From what we read a Fontana-masson should work.  There probably is a
better stain our there for this specific target.  Can anyone with some
experience with staining these granules share their staining protocol.

      At this time the request is not for immuno staining.

      Thanks for your help.

      Nancy Maronto


--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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