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<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=046044117-09102003>What
you are trying to demonstrate are Chromaffin cells of the adrenal glands so a
Masson-Fontana should work, if not try a Schmorl or carry out the Chromaffin
reaction</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=046044117-09102003>If you
use Iodate oxidation you can distinguish between Adrenaline and noradrenalin
fairly easily as iodates oxidise noradrenaline much quicker than they do
adrenaline.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=046044117-09102003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=046044117-09102003>Nick
Kirk</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=046044117-09102003>Histopathology</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=046044117-09102003>Hinchingbrooke Hospital</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=046044117-09102003>Huntingdon</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=046044117-09102003>England</SPAN></FONT></DIV>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B>
histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu]<B>On Behalf Of </B>Nancy
Maronto<BR><B>Sent:</B> 09 October 2003 16:05<BR><B>To:</B>
histonet@lists.utsouthwestern.edu<BR><B>Subject:</B> [Histonet] Need silver
stain for granules in adrenals<BR><BR></FONT></DIV>
<DIV>Hi,</DIV>
<DIV>Could anyone suggest a stain for noradrenalin and adrenalin granules in
paraffin embedded adrenals. We were asked to do a gremilius stain and it
did not stain the granules. Are these slides lost or can we re stain
them? We usually do a recut of the block, but it is asked if we could
use the same tissue slide. The stain actually worked, but not for the
granules.</DIV>
<DIV> </DIV>
<DIV>From what we read a Fontana-masson should work. There probably is a
better stain our there for this specific target. Can anyone with some
experience with staining these granules share their staining protocol.</DIV>
<DIV> </DIV>
<DIV>At this time the request is not for
immuno staining. </DIV>
<DIV> </DIV>
<DIV>Thanks for your help.</DIV>
<DIV> </DIV>
<DIV>Nancy Maronto</DIV></BLOCKQUOTE></BODY></HTML>