[Histonet] Need silver stain for granules in adrenals

Nancy Maronto nancymaronto <@t> sbcglobal.net
Thu Oct 9 10:04:58 CDT 2003


Hi,
Could anyone suggest a stain for noradrenalin and adrenalin granules in paraffin embedded adrenals.  We were asked to do a gremilius stain and it did not stain the granules.  Are these slides lost or can we re stain them?  We usually do a recut of the block, but it is asked if we could use the same tissue slide. The stain actually worked, but not for the granules.
 
>From what we read a Fontana-masson should work.  There probably is a better stain our there for this specific target.  Can anyone with some experience with staining these granules share their staining protocol.
 
At this time the request is not for immuno staining. 
 
Thanks for your help.
 
Nancy Maronto
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