[Histonet] Sentinel nodes

[DeLovino, Salvacion S.]

After the first H&E cut, we cut another for IHC. Then we go 40 microns
deeper then cut another set of H&E/IHC slides. Then we go another 40 microns
deeper and cut the final set. So we have a total of 6 slides. That's if we
have a big enough tissue. If not, then just adjust how deep we go depending
on the size or thickness of the specimen.
 
Salvacion Delovino
 
-----Original Message-----
From: JENNIFER SCHUMACHER [mailto:jschuma1 <@t> FAIRVIEW.ORG] 
Sent: Wednesday, October 08, 2003 1:35 PM
To: histonet <@t> lists.utsouthwestern.EDU
Subject: [Histonet] Sentinel nodes
 
Good afternoon everyone,
We routinely cut our negative sentinel node biopsies shallow, cutting 5
levels (mounting levels 2 and 4 for IHC), approximately 30 microns between
levels.  Now, a pathologist read that "most institutions" are cutting them
deeper, approximately 100 microns between levels.  This is totally an
INFORMAL poll, but I would appreciate any feedback from the community.  I
will check the archives as well, but thought I would get the question out
there before everyone heads off to Kentucky:-)  Thanks.  Jennifer



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