[Histonet] warthin starry

[Carole Fields]

Hi Celia,

We also had problems with gastric bx's and it wasn't a consistent problem.
I retrieved them and then stained them H&E and they were much better.  It
did not seem to matter what you retrieved them with either....very weird.
We think it is something with the fixation.  I had a problem with bone
marrows and it turned out to be, the decal was messed up, but I retrieved
them and they stained better.  Go figure.... but it worked.  My Pathologist
also heard about this at a conference he went to. They did not know why it
worked either.   We do the microwave Steiner now and it works much better.
Try the retrieval...good luck.
Carole Fields
Lexington Medical Center
W.Columbia, SC 

> -----Original Message-----
> From:	celia [SMTP:cfarm <@t> sio.midco.net]
> Sent:	Wednesday, October 08, 2003 6:23 PM
> To:	histonet <@t> lists.utsouthwestern.edu
> Subject:	[Histonet] warthin starry
> 
> Hello Everyone,
>  
> I have put this question out once before and I didn't get much for a
> response so I thought I would try putting it out there one more time.   I
> have an ongoing problem with my Warthin-Starry stain.   The peripheral
> edges of some of the gastric biopsies are not staining.   I am also
> experiencing this same non-staining  with the Steiner stain on the Ventana
> stainer.   I have tried reducing the temp on the processor down from 60c
> to 58c thinking they might be "cooking" on the processor (the H&E's look
> fine)  as well as checking the ph of my solutions.   This did not work.
> Could this be a handling problem prior to fixation in 10% NBF?   I am at
> my wits end.   Any suggestions will be greatly appreciated.
>  
> Thanks in advance.
>  
> Celia, HT
> SVH, Sioux Falls, SD
>  
>  
> 
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