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<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>After the first H&E cut, we cut
another for IHC. Then we go 40 microns deeper then cut another set of H&E/IHC
slides. Then we go another 40 microns deeper and cut the final set. So we have
a total of 6 slides. That's if we have a big enough tissue. If not, then
just adjust how deep we go depending on the size or thickness of the specimen.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><span class=SpellE><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:navy'>Salvacion</span></font></span><font
size=2 color=navy face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:navy'> Delovino<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'>-----Original Message-----<br>
<b><span style='font-weight:bold'>From:</span></b> JENNIFER SCHUMACHER
[mailto:jschuma1@FAIRVIEW.ORG] <br>
<b><span style='font-weight:bold'>Sent:</span></b> Wednesday, October 08, 2003
1:35 PM<br>
<b><span style='font-weight:bold'>To:</span></b>
histonet@lists.utsouthwestern.EDU<br>
<b><span style='font-weight:bold'>Subject:</span></b> [Histonet] Sentinel nodes</span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'><o:p> </o:p></span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'>Good afternoon everyone,<o:p></o:p></span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'>We routinely cut our negative
sentinel node biopsies shallow, cutting 5 levels (mounting levels 2 and 4 for
IHC), approximately 30 microns between levels. Now, a pathologist read
that "most institutions" are cutting them deeper, approximately 100
microns between levels. This is totally an INFORMAL poll, but I would
appreciate any feedback from the community. I will check the archives as
well, but thought I would get the question out there before everyone heads off
to Kentucky:-) Thanks. Jennifer<o:p></o:p></span></font></p>
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