[Histonet] steamers

[Patti Loykasek]

> I agree with Bonnie about preheating the HIER buffer ­ quick & easy. Also, we
> prefer the oval shaped steamer over the round one. The oval one holds more
> water. We do fill it up to the brim!
> 
> Patti Loykasek
> PhenoPath Laboratories
> Seattle, WA 
> 
> 
> I use the B&D too, and I just fill the steamer fuller than you are supposed
> to..... almost to the top, and it always has enough for preheating and the
> longest time I use (40 min).  The other thing I do sometimes (more for speed
> than for the reason you mention) is to heat the buffer in the microwave for a
> bit first.  Then the preheating time is less than 20 minutes.
> Bonnie Whitaker
> University of Texas Medical School at Houston
> 6431 Fannin Street
> MSB 2.231
> Houston, Texas  77030
> Phone 713.500.6792
> Fax 713.500.0733 
>> 
>> -----Original Message-----
>> From: histonet-admin <@t> lists.utsouthwestern.edu
>> [mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Tan, MinHan
>> Sent: Wednesday, December 17, 2003 12:13 PM
>> To: Kim Merriam; Bartlett, Jeanine; histonet <@t> lists.utsouthwestern.edu
>> Subject: RE: [Histonet] steamers
>> 
>> Yes, we use that too. Only problem is that for long ARs (30-35 min), it tends
>> to run out of water if not topped up - it takes about 15-20 min to bring the
>> temp of citrate buffer in a Coplin jar up to 95 deg C.
>>  
>> Min-han Tan
>>  
>>  
>>> -----Original Message-----
>>> From: Kim Merriam [mailto:kmerriam2003 <@t> yahoo.com]
>>> Sent: Wednesday, December 17, 2003 12:04 PM
>>> To: Bartlett, Jeanine; histonet <@t> lists.utsouthwestern.edu
>>> Subject: Re: [Histonet] steamers
>>> 
>>> I just use a Black & Decker Flavor Scenter Handy Steamer.  About 30 bucks at
>>> Walmart!
>>>  
>>> Kim Merriam
>>> Novartis
>>> Cambridge, MA
>>> 
>>> "Bartlett, Jeanine" <jqb7 <@t> cdc.gov> wrote:
>>>> All:
>>>>  
>>>> Can anyone recommend a good, laboratory-grade steamer for antigen
>>>> retrieval?  
>>>>  
>>>> Thanks!
>>>> Jeanine Bartlett, HT(ASCP)
>>>> Centers for Disease Control
>>>> Infectious Disease Pathology Activity
>>>> 1600 Clifton Road, MS/G-32
>>>> Atlanta, GA 30333
>>> 
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>> 
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> 
> 


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