pruegg <@t> colobio.com
Wed Dec 17 13:31:45 CST 2003
I use the steamer and heat the ar buffer in the mw to boiling before putting
it in the steamer that way it only takes about 5 min. to preheat.
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Tan, MinHan
Sent: Wednesday, December 17, 2003 11:13 AM
To: Kim Merriam; Bartlett, Jeanine; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] steamers
Yes, we use that too. Only problem is that for long ARs (30-35 min), it
tends to run out of water if not topped up - it takes about 15-20 min to
bring the temp of citrate buffer in a Coplin jar up to 95 deg C.
From: Kim Merriam [mailto:kmerriam2003 <@t> yahoo.com]
Sent: Wednesday, December 17, 2003 12:04 PM
To: Bartlett, Jeanine; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] steamers
I just use a Black & Decker Flavor Scenter Handy Steamer. About 30 bucks at
"Bartlett, Jeanine" <jqb7 <@t> cdc.gov> wrote:
Can anyone recommend a good, laboratory-grade steamer for antigen retrieval?
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
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