[Histonet] steamers

[Bonnie P Whitaker]

MessageI use the B&D too, and I just fill the steamer fuller than you are
supposed to..... almost to the top, and it always has enough for preheating
and the longest time I use (40 min).  The other thing I do sometimes (more
for speed than for the reason you mention) is to heat the buffer in the
microwave for a bit first.  Then the preheating time is less than 20
minutes.
Bonnie Whitaker
University of Texas Medical School at Houston
6431 Fannin Street
MSB 2.231
Houston, Texas  77030
Phone 713.500.6792
Fax 713.500.0733

  -----Original Message-----
  From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Tan, MinHan
  Sent: Wednesday, December 17, 2003 12:13 PM
  To: Kim Merriam; Bartlett, Jeanine; histonet <@t> lists.utsouthwestern.edu
  Subject: RE: [Histonet] steamers


  Yes, we use that too. Only problem is that for long ARs (30-35 min), it
tends to run out of water if not topped up - it takes about 15-20 min to
bring the temp of citrate buffer in a Coplin jar up to 95 deg C.

  Min-han Tan


    -----Original Message-----
    From: Kim Merriam [mailto:kmerriam2003 <@t> yahoo.com]
    Sent: Wednesday, December 17, 2003 12:04 PM
    To: Bartlett, Jeanine; histonet <@t> lists.utsouthwestern.edu
    Subject: Re: [Histonet] steamers


    I just use a Black & Decker Flavor Scenter Handy Steamer.  About 30
bucks at Walmart!

    Kim Merriam
    Novartis
    Cambridge, MA

    "Bartlett, Jeanine" <jqb7 <@t> cdc.gov> wrote:
      All:

      Can anyone recommend a good, laboratory-grade steamer for antigen
retrieval?

      Thanks!
      Jeanine Bartlett, HT(ASCP)
      Centers for Disease Control
      Infectious Disease Pathology Activity
      1600 Clifton Road, MS/G-32
      Atlanta, GA 30333


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