[Histonet] Citrate Buffer pH

Patsy Ruegg pruegg <@t> colobio.com
Wed Dec 3 12:18:36 CST 2003

Has anybody tried the AR reagents that deparaffinzed such as BioCare
Decloaker in a steamer in place of the pressure cooker?  PC is too harsh for
some of my stuff but I like using these retrieval/deparaffination reagents.

-----Original Message-----
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of
Jackie.O'Connor <@t> abbott.com
Sent: Tuesday, December 02, 2003 2:50 PM
To: Pat Flannery
Cc: Histonet <@t> lists.utsouthwestern.edu;
histonet-admin <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Citrate Buffer pH

I've been using a product from Biocare, a Universal Decloaker (6.0 citrate
buffer) , which deparaffinizes during HIER in their pressure cooker
(Decloaking Chamber) - they are a very Trekkie oriented company.  It works
great - saves a bunch o' steps.  You might try contacting them for the
specifics.  I don't think about the nature of these things if they work - I
just follow along blindly . . . . . . . . .sometimes.

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics

Pat Flannery <pjfnefro <@t> duke.edu>
Sent by: histonet-admin <@t> lists.utsouthwestern.edu
12/02/2003 03:00 PM

        To:        Histonet <@t> lists.utsouthwestern.edu
        Subject:        [Histonet] Citrate Buffer pH

I have a procedure for deparaffinizing (their word, not mine) tissue
sections in order to do immunostaining using the Vector MOM kit.  It
calls for heating the section (briefly) in a microwave in 0.1M Citrate
Buffer.  The procedure  does not specify the pH of the buffer, just to
make it by mixing 0.1M NaCitrate and 0.1M citric acid.  Does this sound
familiar to anyone out there?


-Patrick J. (Pat) Flannery
Division of Nephrology (that's kidneys to you)
Box 3014 (that's NOT "PO" just "Box")
Duke University Medical Center
Durham, NC  27710
E-mail: pjfnefro <@t> duke.edu (preferred)
        FLANN002 <@t> MC.DUKE.EDU (also works)
Voice:   (919)660-6863
Fax:     (919)684-2929

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