[Histonet] Seep macrophage marker

[Patsy Ruegg]

Laurie,
I have done CD68 staining on human, rat, mouse, monkey and rabbit, but have
not tried it on sheep yet.  My cross-reactivity chart lists CD68 as untried
on sheep as well.  One thing that I have found is that the clone PG-M1 is
much more specific to just macrophages and monocytes and not myeloid cells
and a variety of tissues as is clone KP1.  I would not hesitate to try PG-M1
in sheep tissue.  I will contact my local sheep investigators and see if
they have done this.  We have a facility here at UCHSC that just studies
sheep.  Sorry I couldn't be more helpful, keep bugging me to look into this
in case it slips my mind.
Best regards,
Patsy Ruegg

-----Original Message-----
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Laurie Reilly
Sent: Wednesday, November 26, 2003 11:24 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Seep macrophage marker

Dear All,
Does anyone have experience in immuno staining of macrophages in sheep
tissues?
Any help would be appreciated.

         Regards and thanks,   Laurie.

Mr.Laurie Reilly                                              Ph 07 4781
4468
Physiology & Pharmacology                           Fax  07 4779  1526
Aust.Inst.of Tropical Vet.& Animal Sc.
James Cook University
Townsville  Qld.
4811                                      laurie.reilly <@t> jcu.edu.au

Australia.


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