[Histonet] Citrate Buffer pH

Jackie.O'Connor <@t> abbott.com Jackie.O'Connor <@t> abbott.com
Tue Dec 2 15:50:04 CST 2003

I've been using a product from Biocare, a Universal Decloaker (6.0 citrate 
buffer) , which deparaffinizes during HIER in their pressure cooker 
(Decloaking Chamber) - they are a very Trekkie oriented company.  It works 
great - saves a bunch o' steps.  You might try contacting them for the 
specifics.  I don't think about the nature of these things if they work - 
I just follow along blindly . . . . . . . . .sometimes.

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics

Pat Flannery <pjfnefro <@t> duke.edu>
Sent by: histonet-admin <@t> lists.utsouthwestern.edu
12/02/2003 03:00 PM

        To:     Histonet <@t> lists.utsouthwestern.edu
        Subject:        [Histonet] Citrate Buffer pH

I have a procedure for deparaffinizing (their word, not mine) tissue 
sections in order to do immunostaining using the Vector MOM kit.  It 
calls for heating the section (briefly) in a microwave in 0.1M Citrate 
Buffer.  The procedure  does not specify the pH of the buffer, just to 
make it by mixing 0.1M NaCitrate and 0.1M citric acid.  Does this sound 
familiar to anyone out there?


-Patrick J. (Pat) Flannery
 Division of Nephrology (that's kidneys to you)
 Box 3014 (that's NOT "PO" just "Box")
 Duke University Medical Center
 Durham, NC  27710
 E-mail: pjfnefro <@t> duke.edu (preferred)
         FLANN002 <@t> MC.DUKE.EDU (also works)
Voice:   (919)660-6863
Fax:     (919)684-2929

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