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<p class=MsoNormal><span class=EmailStyle16><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;mso-bidi-font-size:12.0pt;font-family:Arial'>Has anybody
tried the AR reagents that deparaffinzed such as BioCare Decloaker in a steamer
in place of the pressure cooker?<span style="mso-spacerun: yes"> </span>PC is
too harsh for some of my stuff but I like using these retrieval/deparaffination
reagents.<o:p></o:p></span></font></span></p>
<p class=MsoNormal><span class=EmailStyle16><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;mso-bidi-font-size:12.0pt;font-family:Arial'>Patsy<o:p></o:p></span></font></span></p>
<p class=MsoNormal><span class=EmailStyle16><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;mso-bidi-font-size:12.0pt;font-family:Arial'><![if !supportEmptyParas]> <![endif]><o:p></o:p></span></font></span></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 color=black
face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;color:black'>-----Original
Message-----<br>
<b><span style='font-weight:bold'>From:</span></b> histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu]<b><span style='font-weight:
bold'>On Behalf Of </span></b>Jackie.O'Connor@abbott.com<br>
<b><span style='font-weight:bold'>Sent:</span></b> Tuesday, December 02, 2003
2:50 PM<br>
<b><span style='font-weight:bold'>To:</span></b> Pat Flannery<br>
<b><span style='font-weight:bold'>Cc:</span></b>
Histonet@lists.utsouthwestern.edu; histonet-admin@lists.utsouthwestern.edu<br>
<b><span style='font-weight:bold'>Subject:</span></b> Re: [Histonet] Citrate
Buffer pH</span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'><![if !supportEmptyParas]> <![endif]><o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-margin-top-alt:0in;margin-right:0in;margin-bottom:
12.0pt;margin-left:.5in'><font size=3 color=black face="Times New Roman"><span
style='font-size:12.0pt;color:black'><br>
</span></font><font size=2 color=black face=Arial><span style='font-size:10.0pt;
font-family:Arial;color:black'>I've been using a product from Biocare, a
Universal Decloaker (6.0 citrate buffer) , which deparaffinizes during HIER in
their pressure cooker (Decloaking Chamber) - they are a very Trekkie oriented
company. It works great - saves a bunch o' steps. You might try
contacting them for the specifics. I don't think about the nature of
these things if they work - I just follow along blindly . . . . . . . .
.sometimes.</span></font><font color=black><span style='color:black'> <br>
<br>
</span></font><font size=2 color=black face=Arial><span style='font-size:10.0pt;
font-family:Arial;color:black'>Jacqueline M. O'Connor HT(ASCP)<br>
Abbott Laboratories<br>
Global Pharmaceutical Research and Development<br>
Discovery Chemotheraputics<br>
</span></font><font color=black><span style='color:black'><br style='mso-special-character:
line-break'>
<![if !supportLineBreakNewLine]><br style='mso-special-character:line-break'>
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windowtext'><o:p></o:p></span></font></p>
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mso-color-alt:windowtext'><o:p></o:p></span></font></p>
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<p class=MsoNormal><b><font size=1 color=black face=sans-serif><span
style='font-size:7.5pt;font-family:sans-serif;color:black;font-weight:bold'>Pat
Flannery <pjfnefro@duke.edu></span></font></b><font color=black><span
style='color:black'> <br>
</span></font><font size=1 color=black face=sans-serif><span
style='font-size:7.5pt;font-family:sans-serif;color:black'>Sent by:
histonet-admin@lists.utsouthwestern.edu</span></font><font color=black><span
style='color:black'> </span></font><font color=black><span style='color:black;
mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p><font size=1 color=black face=sans-serif><span style='font-size:7.5pt;
font-family:sans-serif;color:black'>12/02/2003 03:00 PM</span></font><font
color=black><span style='color:black'> </span></font><font color=black><span
style='color:black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
</td>
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style='font-size:7.5pt;font-family:Arial;color:black'>
</span></font><font color=black><span style='color:black'><br>
</span></font><font size=1 color=black face=sans-serif><span
style='font-size:7.5pt;font-family:sans-serif;color:black'>
To: Histonet@lists.utsouthwestern.edu</span></font><font
color=black><span style='color:black'> <br>
</span></font><font size=1 color=black face=sans-serif><span
style='font-size:7.5pt;font-family:sans-serif;color:black'>
cc: </span></font><font color=black><span
style='color:black'> <br>
</span></font><font size=1 color=black face=sans-serif><span
style='font-size:7.5pt;font-family:sans-serif;color:black'>
Subject: [Histonet] Citrate Buffer pH</span></font><font
color=black><span style='color:black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
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<p class=MsoNormal style='mso-margin-top-alt:0in;margin-right:0in;margin-bottom:
12.0pt;margin-left:.5in'><font size=3 color=black face="Times New Roman"><span
style='font-size:12.0pt;color:black'><br>
<br>
<br>
</span></font><font size=2 color=black face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New";color:black'>I have a
procedure for deparaffinizing (their word, not mine) tissue <br>
sections in order to do immunostaining using the Vector MOM kit. It <br>
calls for heating the section (briefly) in a microwave in 0.1M Citrate <br>
Buffer. The procedure does not specify the pH of the buffer, just
to <br>
make it by mixing 0.1M NaCitrate and 0.1M citric acid. Does this sound <br>
familiar to anyone out there?<br>
<br>
-- <br>
<br>
-Patrick J. (Pat) Flannery<br>
Division of Nephrology (that's kidneys to you)<br>
Box 3014 (that's NOT "PO" just "Box")<br>
Duke University Medical Center<br>
Durham, NC 27710<br>
E-mail: pjfnefro@duke.edu (preferred)<br>
FLANN002@MC.DUKE.EDU (also works)<br>
Voice: (919)660-6863<br>
Fax: (919)684-2929<br>
<br>
<br>
<br>
<br>
_______________________________________________<br>
Histonet mailing list<br>
Histonet@lists.utsouthwestern.edu<br>
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