[Histonet] Bone marrow trephine decalcification

[richard cartun]

 Correct ...... you cannot use acid decal if you want to do molecular testing.  We switched to EDTA for small biopsy specimens that needed decalcifying prior to processing.  It is also important that the specimen be adequately fixed before decalcification.  I am no longer at Hartford Hospital, but I will try to find out where they purchase their EDTA now.  Note that you cannot use EDTA for large specimens (e.g., femoral head) because it takes way too long.  You can also carefully separate all the soft tissue fragments from bone, and process them separately without decalcification.
Richard Cartun

    On Thursday, May 29, 2025 at 04:10:03 AM EDT, MANAHIL EL BIREIR via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 I hope this email finds you well.

I am reaching  out regarding the optimisation of our bone marrow trephine specimen decalcification process. Currently, we are utilizing a ready-to-use acid rapid decalcification method. However, our Molecular Department has observed that the acid affects the quality of molecular results.

To enhance our process and ensure optimal molecular sequencing outcomes, we would greatly appreciate it if you could share your bone marrow trephine decalcification protocol with us. Additionally, which decalcification reagent you use to mitigate potential adverse effects on molecular analysis.

Kind regards,

Manahil


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