[Histonet] Bone marrow trephine decalcification

[Gudrun Lang]

Hi!
For "neutral" decalcification you can mix an 10-20% EDTA solution and add 4%
NaOH to a pH about 7,1-7,4.
There is no difference, if you use the salt or the acid, as long as the pH
is checked.

We fix 3mm-thick bone trephines over night in 4% NBF, then put them for
about 30 hours (8 am one day to 3 pm next day afternoon) in 20% EDTA (pH
7,2). We let it sit on a magnetic stirrer with 40°C to enhance
decalcification. After rinsing in tapwater the cassettes go into
paraffin-processing.

Regards
Gudrun


-----Ursprüngliche Nachricht-----
Von: MANAHIL EL BIREIR via Histonet
[mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Donnerstag, 29. Mai 2025 10:09
An: histonet at lists.utsouthwestern.edu
Betreff: [Histonet] Bone marrow trephine decalcification

I hope this email finds you well.

I am reaching  out regarding the optimisation of our bone marrow trephine
specimen decalcification process. Currently, we are utilizing a ready-to-use
acid rapid decalcification method. However, our Molecular Department has
observed that the acid affects the quality of molecular results.

To enhance our process and ensure optimal molecular sequencing outcomes, we
would greatly appreciate it if you could share your bone marrow trephine
decalcification protocol with us. Additionally, which decalcification
reagent you use to mitigate potential adverse effects on molecular analysis.

Kind regards,

Manahil


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