[Histonet] Ihc control tissues

[Charles Riley]

Awesome thank you Val. I like the multi control option. Can save money in
the end by using less reagents

On Wed, May 8, 2024 at 10:52 AM Val T <vtolley25 at gmail.com> wrote:

> Hey Charles-
>
> Our research lab prepares a “multimouse” control. We embed 10 organs on
> the same slide and it serves as a +/- control for just about every IHC
> marker.
> However, if you don’t want to do that- here are some tissues that would
> make nice controls.
>
> > BCL-2- spleen, lymph nodes
> > Caspase-3- spleen, lymph nodes
> > CD117- skin, spleen, brain, lymph nodes
> > CD62E (e-selectin), bone marrow, colon
> > Collagen II, cartilage
> > Desmin, heart, skeletal muscle
> > GFAP, brain, spinal cord
> > HMB45, skin
> > Ki67, spleen, bowel
> > p53, tumors, colon
> > s100, colon, muscle
>
> Brain can serve as a negative control on just about all these. CD117 will
> have some expression in the cerebellum, but will be negative in most other
> areas.
> Heart can be a negative control for GFAP.
> Val
>
> > On May 8, 2024, at 6:59 AM, Charles Riley via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
> >
> > Hello all,
> >
> > I am trying to put together a positive and negative tissue control list
> for
> > the following antibodies at my research core.  I came over from a
> clinical
> > pathology background and we used tonsils for a lot of these antibodies.
> > Since rats and mice don't have tonsils I am looking for something they do
> > have that can be used if possible to keep the controls as similar to the
> > samples as possible.
> >
> > BCL-2
> > Caspase-3
> > CD117
> > CD62E (e-selectin)
> > Collagen II
> > Desmin
> > GFAP
> > HMB45
> > Ki67
> > p53
> > s100
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