[Histonet] Ihc control tissues

[Val T]

Hey Charles- 

Our research lab prepares a “multimouse” control. We embed 10 organs on the same slide and it serves as a +/- control for just about every IHC marker.
However, if you don’t want to do that- here are some tissues that would make nice controls.

> BCL-2- spleen, lymph nodes
> Caspase-3- spleen, lymph nodes
> CD117- skin, spleen, brain, lymph nodes
> CD62E (e-selectin), bone marrow, colon
> Collagen II, cartilage
> Desmin, heart, skeletal muscle
> GFAP, brain, spinal cord
> HMB45, skin
> Ki67, spleen, bowel
> p53, tumors, colon
> s100, colon, muscle

Brain can serve as a negative control on just about all these. CD117 will have some expression in the cerebellum, but will be negative in most other areas.
Heart can be a negative control for GFAP.
Val

> On May 8, 2024, at 6:59 AM, Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Hello all,
> 
> I am trying to put together a positive and negative tissue control list for
> the following antibodies at my research core.  I came over from a clinical
> pathology background and we used tonsils for a lot of these antibodies.
> Since rats and mice don't have tonsils I am looking for something they do
> have that can be used if possible to keep the controls as similar to the
> samples as possible.
> 
> BCL-2
> Caspase-3
> CD117
> CD62E (e-selectin)
> Collagen II
> Desmin
> GFAP
> HMB45
> Ki67
> p53
> s100
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