[Histonet] Ihc control tissues

[Colleen Forster]

Charles,

I am in a research only lab as well. We create TMA's as control blocks
using the same idea that Val uses. Multiple tissues per block for all IHC
and also good for special stains. You can make nicely organized TMA's
manually very cost effectively as well. Feel free to reach out to me if
interested in more information.

Colleen Forster HT(ASCP)QIHC
cforster at umn.edu

On Wed, May 8, 2024 at 9:56 AM Charles Riley via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Awesome thank you Val. I like the multi control option. Can save money in
> the end by using less reagents
>
> On Wed, May 8, 2024 at 10:52 AM Val T <vtolley25 at gmail.com> wrote:
>
> > Hey Charles-
> >
> > Our research lab prepares a “multimouse” control. We embed 10 organs on
> > the same slide and it serves as a +/- control for just about every IHC
> > marker.
> > However, if you don’t want to do that- here are some tissues that would
> > make nice controls.
> >
> > > BCL-2- spleen, lymph nodes
> > > Caspase-3- spleen, lymph nodes
> > > CD117- skin, spleen, brain, lymph nodes
> > > CD62E (e-selectin), bone marrow, colon
> > > Collagen II, cartilage
> > > Desmin, heart, skeletal muscle
> > > GFAP, brain, spinal cord
> > > HMB45, skin
> > > Ki67, spleen, bowel
> > > p53, tumors, colon
> > > s100, colon, muscle
> >
> > Brain can serve as a negative control on just about all these. CD117 will
> > have some expression in the cerebellum, but will be negative in most
> other
> > areas.
> > Heart can be a negative control for GFAP.
> > Val
> >
> > > On May 8, 2024, at 6:59 AM, Charles Riley via Histonet <
> > histonet at lists.utsouthwestern.edu> wrote:
> > >
> > > Hello all,
> > >
> > > I am trying to put together a positive and negative tissue control list
> > for
> > > the following antibodies at my research core.  I came over from a
> > clinical
> > > pathology background and we used tonsils for a lot of these antibodies.
> > > Since rats and mice don't have tonsils I am looking for something they
> do
> > > have that can be used if possible to keep the controls as similar to
> the
> > > samples as possible.
> > >
> > > BCL-2
> > > Caspase-3
> > > CD117
> > > CD62E (e-selectin)
> > > Collagen II
> > > Desmin
> > > GFAP
> > > HMB45
> > > Ki67
> > > p53
> > > s100
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> > > Histonet at lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
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-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930