[Histonet] [EXT] Reagent alcohols and tissue processors

[Paula Sicurello]

Hi Kara,
I do like the VIPs.   Besides getting rid of the NBF, the alcohols are getting rid of the water as well.
I learned EM first, so my processing thoughts are probably biased by that.
Paula 

Yahoo Mail: Search, Organize, Conquer 
 
  On Thu, Mar 28, 2024 at 6:14 AM, Kara, Phillip<Phillip.Kara at unthsc.edu> wrote:   So for your first one I love the Sakura Tissue-Tek VIP. Easy to use, easy maintenance, and very customizable for programed runs.Every lab I have been in has always started at 70. It saves the techs extra time mixing up dilutions because you can always find RTU 70%. It also helps I have never had any issues with the tissues starting at 70. I get the idea of starting lower but you are also then going to need to increase your processing time which can delay turnaround times.Plus correct me if I am wrong but isn't the whole point of the alcohols after NBF to get the NBF out of the tissue and ready for xylene and wax?

Phillip Kara, HTL | Senior Research Associate

University of North Texas Health Science Center

Division of Research and Innovation 

a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107

p: 918-281-9060

w:www.unthsc.edu/corelabs

From: Paula Sicurello via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, March 28, 2024 8:04 AM
To: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: [EXT] [Histonet] Reagent alcohols and tissue processors Good Morning,
My question two part:
Best tissue processor ever and why?
What is percentage is your first alcohol step?
I see that lots of places start at 70%  but I favor a lower %.  One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%.
Thanks for your insight,
Paula

Yahoo Mail: Search, Organize, Conquer
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cphillip.kara%40unthsc.edu%7Cbb0d8a2788464841512508dc4f27b344%7C70de199207c6480fa318a1afcba03983%7C0%7C0%7C638472279141493958%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=BaFYUI%2FlU5jHIS8xsMkXDj9At%2FONw0U27DhTT6WbYBw%3D&reserved=0