[Histonet] [EXT] Reagent alcohols and tissue processors

[Colleen Forster]

Paula,

I start my processor at 80%. I am in research and often the samples have
been fixed and are put into 70-80% ethanol for holding or transport.
Starting with 80% steps right in line with the samples. I rarely see a
schedule that starts at 50%. However, I do have a couple labs that ask me
for that. I am fortunate to have a second processor I can use for special
programs and not hold up my main runs.

Colleen Forster HT(ASCP)QIHC
University of Minnesota
BLS Histology and IHC Laboratory

On Thu, Mar 28, 2024 at 9:15 AM Paula Sicurello via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi Kara,
> I do like the VIPs.   Besides getting rid of the NBF, the alcohols are
> getting rid of the water as well.
> I learned EM first, so my processing thoughts are probably biased by that.
> Paula
>
> Yahoo Mail: Search, Organize, Conquer
>
>   On Thu, Mar 28, 2024 at 6:14 AM, Kara, Phillip<Phillip.Kara at unthsc.edu>
> wrote:   So for your first one I love the Sakura Tissue-Tek VIP. Easy to
> use, easy maintenance, and very customizable for programed runs.Every lab I
> have been in has always started at 70. It saves the techs extra time mixing
> up dilutions because you can always find RTU 70%. It also helps I have
> never had any issues with the tissues starting at 70. I get the idea of
> starting lower but you are also then going to need to increase your
> processing time which can delay turnaround times.Plus correct me if I am
> wrong but isn't the whole point of the alcohols after NBF to get the NBF
> out of the tissue and ready for xylene and wax?
>
> Phillip Kara, HTL | Senior Research Associate
>
> University of North Texas Health Science Center
>
> Division of Research and Innovation
>
> a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107
>
> p: 918-281-9060
>
> w:www.unthsc.edu/corelabs
>
> From: Paula Sicurello via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Thursday, March 28, 2024 8:04 AM
> To: HistoNet <histonet at lists.utsouthwestern.edu>
> Subject: [EXT] [Histonet] Reagent alcohols and tissue processors Good
> Morning,
> My question two part:
> Best tissue processor ever and why?
> What is percentage is your first alcohol step?
> I see that lots of places start at 70%  but I favor a lower %.  One
> because it's gentler on the tissue, and two because 10% NBF precipitates
> out if you start at 70%.
> Thanks for your insight,
> Paula
>
> Yahoo Mail: Search, Organize, Conquer
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-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930