[Histonet] [EXT] Reagent alcohols and tissue processors

[Kara, Phillip]

So for your first one I love the Sakura Tissue-Tek VIP. Easy to use, easy maintenance, and very customizable for programed runs.
Every lab I have been in has always started at 70. It saves the techs extra time mixing up dilutions because you can always find RTU 70%. It also helps I have never had any issues with the tissues starting at 70. I get the idea of starting lower but you are also then going to need to increase your processing time which can delay turnaround times.
Plus correct me if I am wrong but isn't the whole point of the alcohols after NBF to get the NBF out of the tissue and ready for xylene and wax?


Phillip Kara, HTL | Senior Research Associate

University of North Texas Health Science Center

Division of Research and Innovation

a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107

p: 918-281-9060

w: www.unthsc.edu/corelabs<http://www.unthsc.edu/corelabs>

________________________________
From: Paula Sicurello via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, March 28, 2024 8:04 AM
To: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: [EXT] [Histonet] Reagent alcohols and tissue processors

Good Morning,
My question two part:
Best tissue processor ever and why?
What is percentage is your first alcohol step?
I see that lots of places start at 70%  but I favor a lower %.  One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%.
Thanks for your insight,
Paula

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