[Histonet] Golgi-cox staining

[John Kiernan]

I suggest asking Dr Sami Zaqout, the corresponding author of the paper you cited. A German email address is given on the left side of the first page of the free PDF file for which you provided a web link: https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/ful<https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/full>. He is easy to find with Google and he is now at Qatar University.
It's the most thorough description of Golgi-Cox staining that I've seen, and the pictures of results look superb. Vibratome sectioning evidently is better and quicker than embedding in nitrocellulose and cutting with a sliding  microtome! The two authors' use of  the word "cryoprotectant" was unfortunate in the context of a technique that does not involve freezing.
John Kiernan
Emeritus, Anatomy, University of Western Ontario, Canada
https://www.schulich.uwo.ca/anatomy//people/faculty/emeriti/kiernan_john.html
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________________________________
From: Mariela Chertoff via Histonet <histonet at lists.utsouthwestern.edu>
Sent: April 10, 2024 10:28 AM
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Golgi-cox staining

Hi all

We made the Golgi cox staining and  due to a problem with the vibratome, we
left the tissue several days embedded in  agarose and they get dryed, It is
possoble to recover the brains? it is better to repeat the agarose
embebbing o it is better to put the brains in crioprerervate solution to
rehidrated and after that put them in agarosa again? We are following the
Zaquot protocol
https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/full

Thanks in advance for your reply

Mariela Chertoff, PhD
Laboratorio de Neuroepigenetica - QB75
 Departamento de Química Biológica
Facultad de Ciencias Exactas y Naturales - UBA
Ciudad Universitaria Pabellón II Piso 4
Ciudad Autónoma de Buenos Aires
C1428EGA - Argentina
Tel: 54 11 5285-8680/1/2
email:marielachertoff at gmail.com
marielachertoff at qb.fcen.uba.ar
<mariela.chertoff at uab.cat>
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