[Histonet] Golgi-cox staining

[Mariela Chertoff]

Hi all

We made the Golgi cox staining and  due to a problem with the vibratome, we
left the tissue several days embedded in  agarose and they get dryed, It is
possoble to recover the brains? it is better to repeat the agarose
embebbing o it is better to put the brains in crioprerervate solution to
rehidrated and after that put them in agarosa again? We are following the
Zaquot protocol
https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/full

Thanks in advance for your reply

Mariela Chertoff, PhD
Laboratorio de Neuroepigenetica - QB75
 Departamento de Química Biológica
Facultad de Ciencias Exactas y Naturales - UBA
Ciudad Universitaria Pabellón II Piso 4
Ciudad Autónoma de Buenos Aires
C1428EGA - Argentina
Tel: 54 11 5285-8680/1/2
email:marielachertoff at gmail.com
marielachertoff at qb.fcen.uba.ar
<mariela.chertoff at uab.cat>