[Histonet] FW: Golgi-cox staining

[Bernice Frederick]

From: Rizaldy P Scott <rizaldy.scott at northwestern.edu>
Sent: Wednesday, April 10, 2024 4:33 PM
To: Bernice Frederick <b-frederick at northwestern.edu>
Subject: Re: [Histonet] Golgi-cox staining

Hi Bernice,

What they should have done ideally is to store the uncut agarose blocks submerged in whatever buffer they used to rinse the brains out prior to agarose embedding. They can probably salvage the dried-out brains by rehydrating in their buffer or 30% sucrose solution overnight (or store at 4 degrees as needed) and re-embed in agarose only when they are ready to cut. I can't guarantee however if artefacts due to drying might result but it's worth the shot.


Best regards,
Riz

Rizaldy P. Scott, M.S., Ph.D.
Research Associate Professor of Pathology
Scientific Director
Mouse Histology & Phenotyping Laboratory
Robert H. Lurie Comprehensive Cancer Center
Northwestern University Feinberg School of Medicine
📍Olson Pavilion, Room 8-335, 710 N. Fairbanks Ct., Chicago, IL 60611
✉️  rizaldy.scott at northwestern.edu<mailto:rizaldy.scott at northwestern.edu>
📞 +1-(312)-503-2695
https://www.feinberg.northwestern.edu/sites/mhpl/
www.cancer.northwestern.edu<http://www.cancer.northwestern.edu>


________________________________
From: Bernice Frederick <b-frederick at northwestern.edu<mailto:b-frederick at northwestern.edu>>
Sent: Wednesday, April 10, 2024 13:40
To: Rizaldy P Scott <rizaldy.scott at northwestern.edu<mailto:rizaldy.scott at northwestern.edu>>
Subject: FW: [Histonet] Golgi-cox staining

Can you help this person?
Bernice

-----Original Message-----
From: Mariela Chertoff via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>
Sent: Wednesday, April 10, 2024 9:28 AM
To: histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Golgi-cox staining

Hi all

We made the Golgi cox staining and  due to a problem with the vibratome, we left the tissue several days embedded in  agarose and they get dryed, It is possoble to recover the brains? it is better to repeat the agarose embebbing o it is better to put the brains in crioprerervate solution to rehidrated and after that put them in agarosa again? We are following the Zaquot protocol https://urldefense.com/v3/__https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/full__;!!Dq0X2DkFhyF93HkjWTBQKhk!QkwV4_RJl5cyhElJgSA-8lRMmOSmkwVoHzRrMg8-ZXGX0yUzNCxM_pm09t45KaZWo04kpjD-tBduP59_0DHjxeEvlRMxvl848hoVaCzp$<https://urldefense.com/v3/__https:/www.frontiersin.org/articles/10.3389/fnana.2016.00038/full__;!!Dq0X2DkFhyF93HkjWTBQKhk!QkwV4_RJl5cyhElJgSA-8lRMmOSmkwVoHzRrMg8-ZXGX0yUzNCxM_pm09t45KaZWo04kpjD-tBduP59_0DHjxeEvlRMxvl848hoVaCzp$>

Thanks in advance for your reply

Mariela Chertoff, PhD
Laboratorio de Neuroepigenetica - QB75
 Departamento de Química Biológica
Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabellón II Piso 4 Ciudad Autónoma de Buenos Aires C1428EGA - Argentina
Tel: 54 11 5285-8680/1/2
email:marielachertoff at gmail.com
marielachertoff at qb.fcen.uba.ar<mailto:marielachertoff at qb.fcen.uba.ar>
<mariela.chertoff at uab.cat<mailto:mariela.chertoff at uab.cat>>
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