[Histonet] p17 mice brain sections (Alonso Mart?nez Canabal)

[Amos Brooks]

Hi,
     The thicker the section the more likely it will be to fall off. Frozen
sections already love to fall off slides. You should cut them a lot
thinner. 4 to 10 um should be the thickness, especially for frozens.
     If you want very thick sections as you describe, you would be better
off cutting them and transferring them directly to an 8 well plate with PBS
and doing the IHC as a floating section.

Cheers,
Amos Brooks


>
> Message: 1
> Date: Tue, 19 Sep 2023 19:07:04 -0600
> From: Alonso Mart?nez Canabal <acanabal at ciencias.unam.mx>
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] p17 mice brain sections
> Message-ID:
>         <CAG12gaHPkXVjnHO=
> wDugsDPPX5YTkxXUaipmwctzjcAZwf_d4A at mail.gmail.com>
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>
> Hello,
>    Have a great afternoon. I have done HAR with citrate buffer (pH 6.0)
> with tween, basically all my professional life of some 15 years. Several
> publications, using mice brain sections 40-50 microns thickness from
> cryostat (30% sucrose). Today I tried to do some p17 brain sections and the
> sections did not only fell off, but were completely destroyed.
>       That never happened to me, I am wonder if anyone can have any idea of
> what happened?
>
> Thank you so much
>
> --
> Dr. Alonso Mart?nez Canabal PhD
> Profesor Asociado "C"
> Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM
> Investigador Nacional "I"
> 56224833
>