[Histonet] Jores fluid - Histonet Digest, Vol 238, Issue 10
Anne van Binsbergen
annigyg at gmail.com
Thu Sep 21 12:36:48 CDT 2023
Hello gang
Jores has several references found in a Google search, including a post right here on the Histonet from 2002.
It contains Chloral Hydrate which is very difficult to find.
Do a search and have a look. Several suggestions.
AnnieinArabia (now retired in Africa)
Sent from my iPhone
> On 21 Sep 2023, at 7:07 PM, histonet-request at lists.utsouthwestern.edu wrote:
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> Today's Topics:
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> 1. Detergent in heating antigen retrieval (Alonso Mart?nez Canabal)
> 2. Re: Histonet Digest, Vol 237, Issue 4 (Eddie Martin)
> 3. Re: Long term museum specimen storage (John Kiernan)
> 4. Re: Detergent in heating antigen retrieval (Gudrun Lang)
> 5. p17 mice brain sections (Alonso Mart?nez Canabal) (Amos Brooks)
>
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Wed, 20 Sep 2023 13:24:55 -0600
> From: Alonso Mart?nez Canabal <acanabal at ciencias.unam.mx>
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Detergent in heating antigen retrieval
> Message-ID:
> <CAG12gaFtSo3j4BhVDzf+OXZ7p_tD64t3hFw8+buinJODJzR8zw at mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Dear histoneters,
> I have performed heating antigen retrieval with citrate buffer
> pH 6 with 0.05% tween-20, however I have seem recipes with no detergent,
> anyone has any experience or knowledge if it is better with or without the
> detergent?
> Thank you!
>
> --
> Dr. Alonso Mart?nez Canabal PhD
> Profesor Asociado "C"
> Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM
> Investigador Nacional "I"
> 56224833
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 20 Sep 2023 21:24:14 -0400
> From: Eddie Martin <edmartin26 at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] Histonet Digest, Vol 237, Issue 4
> Message-ID:
> <CADpZgVQw9MMazyhVZN+j0w+x7dOCWhxouG_epVKwJe_qHyRMaA at mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Thought on alternative for Sudan Black. I don't use this stain...an
> alternative is an Oil Red O stain. Oil Red O is done on frozen
> sections...but you can also deparaffinize FFPE sections to water, and then
> perform your Oil Red O stain.
>
> I hope this helps.
>
> Very Respectfully,
> Eddie Martin
>
> Eddie Martin, HTL, QIHC
> The National Institutes of Health
> Bone Marrow Service
> 10 Center Drive
> Building 10, Room 2C360
> Bethesda, MD 20892
> Office: 301-594-2054
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>> On Thu, Aug 10, 2023 at 12:59?PM <histonet-request at lists.utsouthwestern.edu>
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>> 1. Happy Hump Day Histopeeps!! Summer is Almost OVER!!! Here are
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>> (relia1 at earthlink.net)
>> 2. Sudan Black B (Betsy Molinari)
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>> To: <histonet at lists.utsouthwestern.edu>
>> Cc:
>> Bcc:
>> Date: Wed, 9 Aug 2023 14:13:39 -0400
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>> ---------- Forwarded message ----------
>> From: Betsy Molinari <BMolinari at texasheart.org>
>> To: Histonet <Histonet at lists.utsouthwestern.edu>
>> Cc:
>> Bcc:
>> Date: Thu, 10 Aug 2023 14:57:31 +0000
>> Subject: [Histonet] Sudan Black B
>> Hi,
>> I have been asked to do a Sudan stain on a heart biopsy for lipofuscin.
>> The biopsy is in a paraffin block. They are looking to better report and
>> understand the IHC. I am totally unfamiliar with this stain. I did some
>> reading but have been unable to find a protocol for paraffin sections. I
>> found a reference to Sheehan & Hrapchak (1973) but unfortunately I don't
>> have that edition. Any ideas would be greatly appreciated .
>>
>> Betsy Molinari HT (ASCP)
>> Texas Heart Institute
>> Cardiovascular Pathology
>> 1101 Bates St.
>> Houston, Texas 77030
>> 832-355-6524
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>> Betsy Molinari, HT (ASCP)
>> Sr. Histology Research Technician
>> CV Pathology Research
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>> The Texas Heart Institute (r)
>> 6770 Bertner Avenue, MC 1-283
>> Houston, TX 77030
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>> Office: 832-355-6524 | Fax: 832-355-6812
>> Email: BMolinari at texasheart.org
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> ------------------------------
>
> Message: 3
> Date: Thu, 21 Sep 2023 05:20:13 +0000
> From: John Kiernan <jkiernan at uwo.ca>
> To: Histonet <histonet at lists.utsouthwestern.edu>, Rhonda McCormick
> <rmccormick10 at yahoo.com>
> Subject: Re: [Histonet] Long term museum specimen storage
> Message-ID:
> <YT2P288MB0404405B06FABBC543A3AD1BA5F8A at YT2P288MB0404.CANP288.PROD.OUTLOOK.COM>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I don't know anything about "Jore's fixative" or the rationale of using a very hypertonic unbuffered 4% formaldehyde with magnesium, sodium, chloride and sulphate ions. If brown stuff is now bleeding out of your museum specimens, Jore Juice evidently isn't a good preservative.
>
> According to Chapter 26 in the late Charles Culling's excellent book (3rd edn 1974; ISBN: 0407729011) the fixative/preservative for a museum specimen is optimized to preserve colour, which is the red or reddish-brown of haemoglobin and myoglobin. This usually is achieved with Kaiserling's fluid, which contains formalin, potassium acetate and also potassium nitrate (1.5% w/v) as an oxidant. Another approach involves treating specimens with carbon monoxide to convert all haemoglobin etc to a red carboxy derivative.
>
> If your museum specimens have already lost all their meaningful colours, a neutral buffered aqueous formaldehyde may be the best that you can provide to preserve the sizes and shapes. 70% alcohol will cause some shrinkage, and it may not be as easy to seal this solvent into a museum container as a watery diluted formalin.
>
> John Kiernan
> = = =
> ________________________________
> From: Rhonda McCormick via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: September 20, 2023 11:39 AM
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Long term museum specimen storage
>
> Hi All,
> I am looking to replace the fixative for veterinary specimens that have been preserved as "museum specimens". They are kept in jars in a glass case outside our lab, however, some of the fixative is starting to turn brown (and we've pulled a few jars that have some slight cracks in them).
> The specimens are currently in Jore's Fixative: 100 mL Distilled water
> 10 mL 40% Formaldehyde2 g Magnesium Sulfate2 g Sodium Sulfate1 g Sodium Chloride
> Preserving specimens is new to me. I've never heard of Jore's fixative before and I'm wondering if I could get some advice, please? Do these specimens need to be replaced with the same solution? Could we rinse the specimen and replace the solution with 70% Alcohol? OR would 10% NBF be better to store the specimens in (or something al together different)? We have a varying display of specimens - anywhere from a small porcine optic nerve to a large equine granulosa cell tumor. Realizing it may be different based on the size of the specimen, approximately how often should the solution be changed ?
> Thank you so much! Any help or insight is much appreciated.
> Rhonda McCormickRhonda McCormick BS, HT (ASCP)cm
> Histology Diagnostic Lab Supervisor
>
> College of Veterinary Medicine
> Texas A&M University
>
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>
> ------------------------------
>
> Message: 4
> Date: Thu, 21 Sep 2023 13:28:58 +0200
> From: "Gudrun Lang" <gu.lang at gmx.at>
> To: 'Alonso Mart?nez Canabal' <acanabal at ciencias.unam.mx>
> Cc: <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Detergent in heating antigen retrieval
> Message-ID: <001001d9ec7e$d2102c50$763084f0$@gmx.at>
> Content-Type: text/plain; charset="utf-8"
>
> Hi,
> I think the answer is as so often: it depends. Detergens solves membranes partly and leads to a higher permeabilization of the tissue. Some antigens may take advantage of that, some may not need it.
> Higher permeability is good for detection with high molecular complexes.
>
> Gudrun Lang
>
> -----Urspr?ngliche Nachricht-----
> Von: Alonso Mart?nez Canabal via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Gesendet: Mittwoch, 20. September 2023 21:25
> An: Histonet
> Betreff: [Histonet] Detergent in heating antigen retrieval
>
> Dear histoneters,
> I have performed heating antigen retrieval with citrate buffer
> pH 6 with 0.05% tween-20, however I have seem recipes with no detergent,
> anyone has any experience or knowledge if it is better with or without the
> detergent?
> Thank you!
>
> --
> Dr. Alonso Mart?nez Canabal PhD
> Profesor Asociado "C"
> Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM
> Investigador Nacional "I"
> 56224833
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 21 Sep 2023 12:40:40 -0400
> From: Amos Brooks <amosbrooks at gmail.com>
> To: "histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] p17 mice brain sections (Alonso Mart?nez Canabal)
> Message-ID:
> <CAC95ki8n2cLBoatYM5xNktGqOb=kk3T3UdrD1Li-QwpVS_nFWQ at mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Hi,
> The thicker the section the more likely it will be to fall off. Frozen
> sections already love to fall off slides. You should cut them a lot
> thinner. 4 to 10 um should be the thickness, especially for frozens.
> If you want very thick sections as you describe, you would be better
> off cutting them and transferring them directly to an 8 well plate with PBS
> and doing the IHC as a floating section.
>
> Cheers,
> Amos Brooks
>
>
>>
>> Message: 1
>> Date: Tue, 19 Sep 2023 19:07:04 -0600
>> From: Alonso Mart?nez Canabal <acanabal at ciencias.unam.mx>
>> To: Histonet <histonet at lists.utsouthwestern.edu>
>> Subject: [Histonet] p17 mice brain sections
>> Message-ID:
>> <CAG12gaHPkXVjnHO=
>> wDugsDPPX5YTkxXUaipmwctzjcAZwf_d4A at mail.gmail.com>
>> Content-Type: text/plain; charset="UTF-8"
>>
>> Hello,
>> Have a great afternoon. I have done HAR with citrate buffer (pH 6.0)
>> with tween, basically all my professional life of some 15 years. Several
>> publications, using mice brain sections 40-50 microns thickness from
>> cryostat (30% sucrose). Today I tried to do some p17 brain sections and the
>> sections did not only fell off, but were completely destroyed.
>> That never happened to me, I am wonder if anyone can have any idea of
>> what happened?
>>
>> Thank you so much
>>
>> --
>> Dr. Alonso Mart?nez Canabal PhD
>> Profesor Asociado "C"
>> Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM
>> Investigador Nacional "I"
>> 56224833
>>
>
>
> ------------------------------
>
> Subject: Digest Footer
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>
> End of Histonet Digest, Vol 238, Issue 10
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