[Histonet] Histonet Digest, Vol 237, Issue 4

Wed Sep 20 20:24:14 CDT 2023

Thought on alternative for Sudan Black.  I don't use this stain...an
alternative is an Oil Red O stain.  Oil Red O is done on frozen
sections...but you can also deparaffinize FFPE sections to water, and then
perform your Oil Red O stain.

I hope this helps.

Very Respectfully,
Eddie Martin

Eddie Martin, HTL, QIHC
The National Institutes of Health
Bone Marrow Service
10 Center Drive
Building 10, Room 2C360
Bethesda, MD 20892
Office: 301-594-2054

On Thu, Aug 10, 2023 at 12:59 PM <histonet-request at lists.utsouthwestern.edu>
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>    1. Happy Hump Day Histopeeps!! Summer is Almost OVER!!!      Here are
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>    2. Sudan Black B (Betsy Molinari)
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> ---------- Forwarded message ----------
> From: Betsy Molinari <BMolinari at texasheart.org>
> To: Histonet <Histonet at lists.utsouthwestern.edu>
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> Bcc:
> Date: Thu, 10 Aug 2023 14:57:31 +0000
> Subject: [Histonet] Sudan Black B
> Hi,
> I have been asked to do a Sudan stain on a heart  biopsy for lipofuscin.
> The biopsy is in a paraffin block. They are looking  to better report and
> understand the IHC. I am totally unfamiliar with this stain. I did some
> reading but have been unable to find a protocol for paraffin sections. I
> found a reference to Sheehan & Hrapchak (1973) but unfortunately I don't
> have that edition.  Any ideas would be greatly appreciated .
>
> Betsy Molinari HT (ASCP)
> Texas Heart Institute
> Cardiovascular Pathology
> 1101 Bates St.
> Houston, Texas  77030
> 832-355-6524
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> Betsy Molinari, HT (ASCP)
> Sr. Histology Research Technician
> CV Pathology Research
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> The Texas Heart Institute (r)
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