[Histonet] Detergent in heating antigen retrieval

[Gudrun Lang]

Hi,
I think the answer is  as so often: it depends. Detergens solves membranes partly and leads to a higher permeabilization of the tissue. Some antigens may take advantage of that, some may not need it. 
Higher permeability is good for detection with high molecular complexes. 

Gudrun Lang

-----Ursprüngliche Nachricht-----
Von: Alonso Martínez Canabal via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Mittwoch, 20. September 2023 21:25
An: Histonet
Betreff: [Histonet] Detergent in heating antigen retrieval

Dear histoneters,
           I have performed heating antigen retrieval with citrate buffer
pH 6 with 0.05% tween-20, however I have seem recipes  with no detergent,
anyone has any experience or knowledge if it is better with or without the
detergent?
          Thank you!

-- 
Dr. Alonso Martínez Canabal PhD
Profesor Asociado "C"
Departamento de Biología Celular, Facultad de Ciencias, UNAM
Investigador Nacional "I"
56224833
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