[Histonet] Sudanblack B on FFPET

[John Kiernan]

You probably would get better results with a supersaturated solution of the dye in 60% isopropanol with added dextrin. For details see https://www.urmc.rochester.edu/urmc-labs/pathology/StainsManual/. Click on Table of Contents and follow the links to fined the method. Be sure to use a batch of Sudan black B certified by the Biological Stain Commission.
John Kiernan
London, Canada
= = =
________________________________
From: Gudrun Lang via Histonet <histonet at lists.utsouthwestern.edu>
Sent: March 28, 2023 10:30 AM
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Sudanblack B on FFPET

Hallo!

Has anyone experience with Sudanblack B on paraffin slides for staining
lipofuszin? A doctor wants the demonstration of the lipoid content of foamy
cells or granulocytes in lung.



I've found protocols that have incubation-times from 10 minutes to
over-night.

I've made a saturated Sudanblack B-solution in 70% ethanol and tried it with
10 min on liver without real success.



I would appreciate any input and help.

Thanks in advance



Gudrun Lang

Austria

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet