[Histonet] Sudanblack B on FFPET

[Gudrun Lang]

Hallo!

Has anyone experience with Sudanblack B on paraffin slides for staining
lipofuszin? A doctor wants the demonstration of the lipoid content of foamy
cells or granulocytes in lung.



I've found protocols that have incubation-times from 10 minutes to
over-night.

I've made a saturated Sudanblack B-solution in 70% ethanol and tried it with
10 min on liver without real success.



I would appreciate any input and help.

Thanks in advance



Gudrun Lang

Austria