[Histonet] Alcian blue van gieson double stain

[Paula Keene Pierce]

Hi,
if they are wanting to stain elastic, Alcian blue, and Van Gieson, I recommend doing the Verhoff elastic stain first, then the Alcian blue, and then the Van Gieson.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

    On Thursday, March 9, 2023 at 01:05:14 PM CST, Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 I am working with a researcher and they saw an article where someone
performed an Alcian Blue  and elastic van Gieson double stain.  If anyone
has done this before can you provide your protocol.  I am trying to figure
out the best way to perform this process to get the best staining results.
Here are my questions/thoughts

1. Is it better to run the Alcian blue as usual and then perform the van
gieson stain?
2. Will the differentiating solution in the van gieson stain affect the
alcian blue staining in any way?
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