[Histonet] Alcian blue van gieson double stain

Charles Riley criley at udel.edu
Thu Mar 9 13:00:23 CST 2023


I am working with a researcher and they saw an article where someone
performed an Alcian Blue  and elastic van Gieson double stain.  If anyone
has done this before can you provide your protocol.  I am trying to figure
out the best way to perform this process to get the best staining results.
Here are my questions/thoughts

1. Is it better to run the Alcian blue as usual and then perform the van
gieson stain?
2. Will the differentiating solution in the van gieson stain affect the
alcian blue staining in any way?


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