[Histonet] Alcian blue van gieson double stain

[Akemi Allison]

Just curious, why not do a Movat’s Pentachome? It gives you everything you are looking for and more.  
Best regards,
Akemi Allison-Tacha, BS, Ht, HTL 
Akemiat3377 at gmail.com

Sent from my iPhone

> On Mar 9, 2023, at 11:19 AM, Paula Keene Pierce via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Hi,
> if they are wanting to stain elastic, Alcian blue, and Van Gieson, I recommend doing the Verhoff elastic stain first, then the Alcian blue, and then the Van Gieson.
> Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com
> 
> A sharp knife is nothing without a sharp eye. - Klingon Proverb 
> 
>    On Thursday, March 9, 2023 at 01:05:14 PM CST, Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
> 
> I am working with a researcher and they saw an article where someone
> performed an Alcian Blue  and elastic van Gieson double stain.  If anyone
> has done this before can you provide your protocol.  I am trying to figure
> out the best way to perform this process to get the best staining results.
> Here are my questions/thoughts
> 
> 1. Is it better to run the Alcian blue as usual and then perform the van
> gieson stain?
> 2. Will the differentiating solution in the van gieson stain affect the
> alcian blue staining in any way?
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