[Histonet] A question for my hospital lab peeps

[Samantha Golden]

If a SLN is sent for frozen section (boo hiss) we treat as any other frozen and we typically cut two sections. If the pathologist is able to determine the lymph node is positive, we will only cut a permanent H&E once processed; we do not need to cut all the SLN levels. If the tissue appears negative on frozen then we will treat it as a routine SLN; we then cut the permanent H&E and an unstain, trim in about 100 um, cut an H&E and an unstain, trim in about 100 um and cut the final H&E. Hope this makes sense!

Samantha 
Sent from my iPhone

> On Feb 23, 2023, at 1:38 PM, Terri Braud via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> I'd love to hear your input
> On breast mastectomy cases with sentinel lymph nodes, what is your cutting protocol for sentinel lymph nodes sent for frozen section?
> How many frozen levels?
> How many permanent section Levels?
> Any unstained ffpe sections saved or stained IHC?
> I'm just trying to make sure that what we're doing is the norm.  Thanks in advance.
> 
> Terri L. Braud, HT(ASCP)
> HNL Laboratories for 
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> Ph: 215-938-3689
> Fax: 215-938-2021
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