[Histonet] A question for my hospital lab peeps

[Cone, Merry A]

Hi Terri!

At the hospital I'm currently at, we do the same as Lacy's lab for permanent and frozen sections.  We also wait for the pathologists to put in IHC requests before cutting them and they will also often request a follow up H&E.  I have worked at another facility that would a 'level 1' and 'level 100' on all sentinel nodes without any IHC up front.

Hope that helps!
Amber Cone, HTL(CM)

-----Original Message-----
From: Normington, Lacy <LNormington at uwhealth.org> 
Sent: Thursday, February 23, 2023 12:56 PM
To: Terri Braud <tbraud at holyredeemer.com>; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] A question for my hospital lab peeps

Hello Terri,
Please see an excerpt from our frozen section manual.

For all sentinel lymph nodes and non-sentinel lymph nodes obtained from breast and gynecologic surgeries, entirely submit the lymph node after serially sectioning perpendicular to the long axis at 0.2 cm intervals. It is essential that all unique surfaces be represented, i.e.
opposing surfaces should not be embedded in the same direction.

How many frozen levels? Seeing as we are serially sectioning the lymph node, we only do a single level.
How many permanent section Levels? All remnants are submitted after frozen, again we only perform the microtomy of a single level.
Any unstained ffpe sections saved or stained IHC? It is not uncommon for AE1/AE3 to be requested which we then cut the IHC request followed by an additional recut H&E.

Thanks
Lacy Normington

Lacy Normington, HTL(CM)
She/Her/Hers
Manager, Clinical Laboratories
Surgical Pathology, Autopsy
600 Highland Ave, Mail 3224
Madison, WI  53792
Office: 608.890.9373


-----Original Message-----
From: Terri Braud via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, February 23, 2023 12:17 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] A question for my hospital lab peeps

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I'd love to hear your input
On breast mastectomy cases with sentinel lymph nodes, what is your cutting protocol for sentinel lymph nodes sent for frozen section?
How many frozen levels?
How many permanent section Levels?
Any unstained ffpe sections saved or stained IHC?
I'm just trying to make sure that what we're doing is the norm.  Thanks in advance.

Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-2021
          Honesty
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