[Histonet] destain for IHC

[RENE MORALES]

Nancy, I would say that it can be done in a very particular situation: i.e.: no more tissue available, and type of IHC stain. If it is a dual stain, pathologist can not obtain the good results. In the lab I worked before, it was done, but this technique was not part of the protocol.
Hope it helps,
René 

Sent from my iPhone

> On Feb 8, 2023, at 10:35, Bacon, Charles via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Hi Nancy,
> 
> We use our Acid alcohol solution from our AFB Stain. It pulls off the Eosin and leaves a faded Hematoxylin behind. We counterstain in Hematoxylin anyway so we find this protocol effective. Then we remove the deparaffinization step from our IHC protocol and run the slide.
> 
> 1% Acid Alcohol
> 70% Alcohol......99ml
> HCL...................1ml
> 
> Chuck Bacon, HTL(ASCP)CM 
> Supervisor Histology
> Baystate Medical Center
> 361 Whitney Ave., Holyoke, MA 01040
> Telephone: 413-322-4786  Fax: 413-322-4790
> Charles.Bacon at baystatehealth.org
> 
> -----Original Message-----
> From: Cooper, Brian <bcooper at chla.usc.edu> 
> Sent: Wednesday, February 8, 2023 12:46 PM
> To: Nancy Schmitt <Nancy.Schmitt at mercyhealth.com>; Histonet <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] destain for IHC
> 
> We used to do this all the time in my old reference lab.
> 
> 
> Thanks,
> 
> 
> 
> Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
> 
> Department of Pathology and Laboratory Medicine
> 
> Children's Hospital Los Angeles
> 
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
> 
> Ph: 323.361.3357<tel:+13233613357>
> 
> bcooper at chla.usc.edu<mailto:bcooper at chla.usc.edu>
> 
> ________________________________
> From: Nancy Schmitt via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Wednesday, February 8, 2023 9:29:13 AM
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] destain for IHC (EXTERNAL EMAIL)
> 
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> 
> Hello-
> 
> Has anyone had success destaining an H&E and then running IHC on the same slide?
> 
> Thanks!
> 
> Nancy
> 
> 
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