[Histonet] destain for IHC

[Gudrun Lang]

We do restaining for IHC also, but without destaining at all. Hematoxylin
doesn't matter because nuclei are stained after IHC with hematoxylin anyway.
And Eosin gets lost through all the washing in buffer.
The problem is, that HE section are usually mounted on non-adhesive slides,
and they float away during the procedure.
Gudrun

-----Ursprüngliche Nachricht-----
Von: Nancy Schmitt via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Mittwoch, 8. Februar 2023 18:29
An: Histonet
Betreff: [Histonet] destain for IHC

Hello-

Has anyone had success destaining an H&E and then running IHC on the same
slide?

Thanks!

Nancy


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