[Histonet] maximum thickness brain in slide

[Colleen Forster]

Alonso,

For sections that thick you would collect them as you always have, stain
them as floating sections and then mount onto a glass slide. The routine
standard methods for IHC will never penetrate a  50um thick section.

Colleen Forster HT(ASCP)QIHC



On Fri, Aug 4, 2023 at 1:17 PM Alonso Martínez Canabal via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Good afternoon Histoneters,
>        I have been doing IHC and IF during decades in rodent brain tissue,
> always in free floating. Now, I would like to do that sections placed over
> slides and perform the whole procedure over the slide. I am interested in
> using thick sections, usually I used 50micrometers, what is the maximum
> thickness that I could use in slide to guarantee the correct penetration of
> the antibodies and the ABC complex. Before the IHC I always use graded
> alcohols and xilene, and antigen retrieval with citrate buffer.
>       Thank you!
>
> --
> Dr. Alonso Martínez Canabal PhD
> Profesor Asociado "C"
> Departamento de Biología Celular, Facultad de Ciencias, UNAM
> Investigador Nacional "I"
> 56224833
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>


-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930