[Histonet] maximum thickness brain in slide

[Alonso Martínez Canabal]

Hi, thank you for your reply.
    I cannot do my usual free floating staining because this tissue is very
brittle. So, I was thinking to do the staining in slide at 30 microns?
Thank you

El vie, 4 ago 2023 a las 12:29, Colleen Forster (<cforster at umn.edu>)
escribió:

> Alonso,
>
> For sections that thick you would collect them as you always have, stain
> them as floating sections and then mount onto a glass slide. The routine
> standard methods for IHC will never penetrate a  50um thick section.
>
> Colleen Forster HT(ASCP)QIHC
>
>
>
> On Fri, Aug 4, 2023 at 1:17 PM Alonso Martínez Canabal via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
>> Good afternoon Histoneters,
>>        I have been doing IHC and IF during decades in rodent brain tissue,
>> always in free floating. Now, I would like to do that sections placed over
>> slides and perform the whole procedure over the slide. I am interested in
>> using thick sections, usually I used 50micrometers, what is the maximum
>> thickness that I could use in slide to guarantee the correct penetration
>> of
>> the antibodies and the ABC complex. Before the IHC I always use graded
>> alcohols and xilene, and antigen retrieval with citrate buffer.
>>       Thank you!
>>
>> --
>> Dr. Alonso Martínez Canabal PhD
>> Profesor Asociado "C"
>> Departamento de Biología Celular, Facultad de Ciencias, UNAM
>> Investigador Nacional "I"
>> 56224833
>> _______________________________________________
>> Histonet mailing list
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>>
>
>
> --
> Colleen Forster HT(ASCP)QIHC
> BLS Histology and IHC Laboratory
> Jackson Hall, Room 2-155
> 321 Church St. SE
> Minneapolis, MN 55455
> 612-626-1930
>
>
>
>
>
>
>

-- 
Dr. Alonso Martínez Canabal PhD
Profesor Asociado "C"
Departamento de Biología Celular, Facultad de Ciencias, UNAM
Investigador Nacional "I"
56224833