[Histonet] IHC background

[RENE MORALES]

Paula,
Regardless of the position of the tissue: control/ patient ‘s tissue, most likely is the  distribution of the reagents during staining, isn’t adequate. Syringe, staining  reagents expiration, calibration, etc.,   Have you contacted service from Leica? Hope the solution is found,
Thanks,


Sent from my iPhone

> On Sep 29, 2022, at 12:00, Paula via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Hello everyone,
> 
> 
> 
> We have the Bond III and we ran an S100.  We placed 2 serial sections
> vertically on the slide with the control tissue at the top for staining. The
> patient's section at the top underneath the control tissue had background
> staining, while the section on the bottom of that had no background
> staining.  Can someone determine why this would happen?
> 
> 
> 
> Thank you in advance.
> 
> Paula
> 
> Bio-Path Medical Group
> 
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