[Histonet] IHC background

[Regan Fulton]

Hi,

The artifact you are seeing is a well-known artifact of the Leica covertile
design.
It does limit the available footprint for placing samples.

Hope this helps.

Regan



Regan Fulton, M.D., Ph.D.
CEO and Co-Founder
Array Science, LLC
475 Gate 5 Road, #100
Sausalito, CA 94965
(415) 577-7360



www.arrayscience.com



On Thu, Sep 29, 2022 at 12:37 PM RENE MORALES via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Paula,
> Regardless of the position of the tissue: control/ patient ‘s tissue, most
> likely is the  distribution of the reagents during staining, isn’t
> adequate. Syringe, staining  reagents expiration, calibration, etc.,   Have
> you contacted service from Leica? Hope the solution is found,
> Thanks,
>
>
> Sent from my iPhone
>
> > On Sep 29, 2022, at 12:00, Paula via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
> >
> > Hello everyone,
> >
> >
> >
> > We have the Bond III and we ran an S100.  We placed 2 serial sections
> > vertically on the slide with the control tissue at the top for staining.
> The
> > patient's section at the top underneath the control tissue had background
> > staining, while the section on the bottom of that had no background
> > staining.  Can someone determine why this would happen?
> >
> >
> >
> > Thank you in advance.
> >
> > Paula
> >
> > Bio-Path Medical Group
> >
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>
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