[Histonet] IHC background

[Paula]

Hello everyone,

 

We have the Bond III and we ran an S100.  We placed 2 serial sections
vertically on the slide with the control tissue at the top for staining. The
patient's section at the top underneath the control tissue had background
staining, while the section on the bottom of that had no background
staining.  Can someone determine why this would happen?

 

Thank you in advance.

Paula

Bio-Path Medical Group