[Histonet] IHC staining of cartilage

[Kurth, Virginia L]

Make sure they sit at room temperature longer than normal and then dry them longer.  I found that cutting them thinner (3 microns) also helps. 



Ginny Kurth, HT, (ASCP)CM
UW Health Surgical Pathology
1111 Highland Ave
Madison, WI 






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From: Hobbs, Carl via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Tuesday, March 01, 2022 1:30 PM
To: histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] IHC staining of cartilage

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Yes...a big problem for me, too....if you are using HIER??
Cartilage and bone are major tissue retention problems I cut Pwax sections of mouse long bones/knee joints for anti GFP IHC I still get a % fall off but,  using manual HIER at 90C I manage to get enough sections to get good results However, I imagine that your autoIHC stainer prob uses 90C HIER?
I find Trajan 3 series slides more reliable than Superfrost Plus slides.
Sure, I need to dry my slides for 24 hrs in an operating fume hood ( we can't turn ours off/down) Then I place slides in a 60C oven for 2hrs.
Then dewax etc....

Be interesting to read of other suggestions

Best wishes

Carl

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL



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