[Histonet] IHC staining of cartilage

[Regan Fulton]

Hi,

With regard to adhesion, we find TOMO slides are the best among many that
we have tried.

Interestingly, a creative histotech I once worked with used a diluted
Gorilla Glue smear on the slides just prior to picking up a cartilage
section.  It was tricky to get the section on while the glue was in a
workable condition, but the adhesion was phenomenally improved.  IHC worked
very well on these slides.

Best regards,

Regan


Regan Fulton, M.D., Ph.D.
CEO and Co-Founder
Array Science, LLC
475 Gate 5 Road, #100
Sausalito, CA 94965
(415) 577-7360



www.arrayscience.com



On Tue, Mar 1, 2022 at 11:30 AM Hobbs, Carl via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Yes...a big problem for me, too....if you are using HIER??
> Cartilage and bone are major tissue retention problems
> I cut Pwax sections of mouse long bones/knee joints for anti GFP IHC
> I still get a % fall off but,  using manual HIER at 90C I manage to get
> enough sections to get good results
> However, I imagine that your autoIHC stainer prob uses 90C HIER?
> I find Trajan 3 series slides more reliable than Superfrost Plus slides.
> Sure, I need to dry my slides for 24 hrs in an operating fume hood ( we
> can't turn ours off/down)
> Then I place slides in a 60C oven for 2hrs.
> Then dewax etc....
>
> Be interesting to read of other suggestions
>
> Best wishes
>
> Carl
>
> Carl Hobbs FIBMS
> Histology and Imaging Manager
> Wolfson CARD
> Guys Campus, London Bridge
> Kings College London
> London
> SE1 1UL
>
>
>
> 020 7848 6810
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