[Histonet] Using LR white with a tungsten carbide knife

Chaitanya Kolluru cxk340 at case.edu
Tue Oct 5 13:17:40 CDT 2021


I’m looking for some advice on using LR white on an osmium stained tissue.
Unfortunately, I don’t have access to an ultra-microtome or diamond knives.

Has someone here tried sectioning LR white with tungsten carbide knives on
a motorized rotary microtome? Are there any sectioning artifacts that I
might see? Also, LR white seems to be available in three grades  - soft,
medium and hard. Is the soft grade recommended with my setup?

Thank you,

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