[Histonet] Histonet Digest, Vol 215, Issue 3

Tue Oct 5 12:35:37 CDT 2021

If you are looking to prioritize certain tissues slides to the pathologist,
I think separate small biopsy runs are a great idea. You can process them
with a much shorter run cycle.(90-120 min). You can actually do several
runs per day. This is a good way of decreasing your TAT for all blocks and
putting the signout TAT back in the pathologist's hands. If you are
interested, I can suggest a run schedule.

*John Frazier*
*MT(ASCP), MBA, *
*Lean 6 Six Sigma Black Belt*
*Healthcare Consultant*
*C - 302-310-7567                *
*H - 704-847-0566*
*wilfong1923 at gmail.com <wilfong1923 at gmail.com>*

On Tue, Oct 5, 2021 at 1:00 PM <histonet-request at lists.utsouthwestern.edu>
wrote:

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>    1. Re: Dissect Aid - Histonet Digest, Vol 215, Issue 2 (Terri  Braud)
>    2. Fixative in diff-quick (Corbin, Clay)
>    3. Re: Fixative in diff-quick (Bryan Llewellyn)
>    4. Tissue Quality (Chakib Boussahmain)
>    5. microtome (Silvia Bonner)
>    6. FW: Biopsy processing (Hannen, Valerie)
>
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> ---------- Forwarded message ----------
> From: Terri  Braud <tbraud at holyredeemer.com>
> To: "histonet at lists.utsouthwestern.edu" <histonet at lists.utsouthwestern.edu
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> Cc:
> Bcc:
> Date: Mon, 4 Oct 2021 17:48:32 +0000
> Subject: Re: [Histonet] Dissect Aid - Histonet Digest, Vol 215, Issue 2
> We do not re-use.
>
> Terri L. Braud, HT(ASCP)
> HNL Laboratories for
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
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> -----Original Message-----
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>    1. dissect aid (Nancy Schmitt)
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Mon, 4 Oct 2021 15:16:31 +0000
> From: Nancy Schmitt <Nancy.Schmitt at mercyhealth.com>
> To: "histonet at lists.utsouthwestern.edu"
>         <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] dissect aid
> Message-ID:
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> Happy Monday!
> For those using Dissect Aid (Statlab) - are you re-using this product for
> multiple specimens?
> Can you please share what your process is for this?
> Much appreciated,
> Nancy Schmitt MLT, HT(ASCP)
> MercyOne Dubuque
>
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> End of Histonet Digest, Vol 215, Issue 2
> ****************************************
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>
>
> ---------- Forwarded message ----------
> From: "Corbin, Clay" <ccorbin at bloomu.edu>
> To: "histonet at lists.utsouthwestern.edu" <histonet at lists.utsouthwestern.edu
> >
> Cc:
> Bcc:
> Date: Mon, 4 Oct 2021 20:58:17 +0000
> Subject: [Histonet] Fixative in diff-quick
> Hey folks,
> I am shopping for a diff-quick kit.  However, all I really need is the
> fixative.  Generally, there is a blue stain (triarylmethane) added to the
> methanol in the fixative solution.  I have a giant jug of lab grade
> methanol.  What would I lose by using methanol alone compared to the
> fixative solution included in a diff-quick kit?
> Thanks!
> Clay
>
> Clay Corbin, PhD
> Professor of Biology
> Bloomsburg University
>
>
>
> ---------- Forwarded message ----------
> From: Bryan Llewellyn <llewllew at shaw.ca>
> To: Clay <ccorbin at bloomu.edu>, Histonet <histonet at lists.utsouthwestern.edu
> >
> Cc:
> Bcc:
> Date: Mon, 4 Oct 2021 14:40:53 -0700
> Subject: Re: [Histonet] Fixative in diff-quick
> Diff Quick appears to be a modified Field's stain. The blue dye in the
> methanol is one of the modifications. Field's stain gives much the same
> staining, so using plain methanol should be of no concern. The simplest
> way to find out is surely to try it and see.
>
> http://stainsfile.info/stain/micro/field.htm
>
> Bryan Llewellyn
>
>
> Corbin, Clay via Histonet wrote:
> > Hey folks,
> > I am shopping for a diff-quick kit.  However, all I really need is the
> fixative.  Generally, there is a blue stain (triarylmethane) added to the
> methanol in the fixative solution.  I have a giant jug of lab grade
> methanol.  What would I lose by using methanol alone compared to the
> fixative solution included in a diff-quick kit?
> > Thanks!
> > Clay
> >
> > Clay Corbin, PhD
> > Professor of Biology
> > Bloomsburg University
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> > Histonet at lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>
>
>
>
>
> ---------- Forwarded message ----------
> From: Chakib Boussahmain <chak_bou at yahoo.com>
> To: "histonet at lists.utsouthwestern.edu" <histonet at lists.utsouthwestern.edu
> >
> Cc:
> Bcc:
> Date: Tue, 5 Oct 2021 12:49:47 +0000 (UTC)
> Subject: [Histonet] Tissue Quality
>
> Hi Guys,
>
> We normally buy human tissue blocks to use them as controlsor to develop
> IF assays in our laboratory. However, most of these blocksappeared to be
> fixed or processed adequately. I am wondering if anybody has amethod to
> assess the quality of these tissue blocks besides H&E stain. Forinstance,
> is there an IHC marker or a panel of the markers that I can use as agood
> indicator to qualify these tissue blocks?
>
> Thank you so much in advance for any suggestions!
>
> Best
>
> Chakib
>
>
>
>
>
> ---------- Forwarded message ----------
> From: Silvia Bonner <sbonner at pathregional.com>
> To: "histonet at lists.utsouthwestern.edu" <histonet at lists.utsouthwestern.edu
> >
> Cc:
> Bcc:
> Date: Tue, 5 Oct 2021 16:19:29 +0000
> Subject: [Histonet] microtome
> Hello,
>
> We are in the market for a new/refurbished microtome.  We currently have a
> Micron HM355s, and Micron HM 335E.  We are interested in buying something
> comparable.  Any suggestions?  We are looking at Micron and Leica.  Good or
> bad reviews would be appreciated.
>
> Thanks,
>
>
> Silvia Bonner, BS, HT(ASCP) CM
>
> Histology Supervisor
>
>
>
> sbonner at pathregional.com<mailto:sbonner at pathregional.com>
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> ---------- Forwarded message ----------
> From: "Hannen, Valerie" <Valerie.Hannen at parrishmed.com>
> To: "Histonet at lists.utsouthwestern.edu" <Histonet at lists.utsouthwestern.edu
> >
> Cc:
> Bcc:
> Date: Tue, 5 Oct 2021 16:30:46 +0000
> Subject: [Histonet] FW: Biopsy processing
>
>
> From: Hannen, Valerie
> Sent: Monday, October 04, 2021 2:54 PM
> To: 'histonet at list.utsouthwestern.edu'
> Subject: FW: Biopsy processing
>
>
>
> From: Hannen, Valerie
> Sent: Monday, October 4, 2021 11:03 AM
> To: 'histonet at list.utsouthwestern.edu' <histonet at list.utsouthwestern.edu>
> Subject: Biopsy processing
>
>   Good Morning All...      My Pathologist is wondering if there is any
> benefit to having a separate processing run just for small biopsies?  He
> said that what is seeing now, he has no issue with, the sections are just
> as good as all the other tissue sections. He just asked for me to get the
> consensus of the group.
>
> Thank You in advance!!
>
>
>
>
> Valerie A. Hannen,MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Avenue
> Titusville, Florida 32796
> P: 321-268-6333  Ext. 7506
> F: 321-268-6149
> valerie.hannen at parrishmed.com<mailto:valerie.hannen at parrishmed.com>
> www.parrishmed.com<http://www.parrishmed.com>
>
>
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