[Histonet] Fw: Fixative in diff-quick

Tony Henwood (SCHN) tony.henwood at health.nsw.gov.au
Tue Oct 5 14:17:01 CDT 2021

Hi Clay,

The first solution is methanol.
So lab grade methanol will do.
The blue colour is from an innocuous dye added to differentiate from water (and ethanol used in cytology fixation). This helps us poor cytologists when we are doing ROSE at FNAs.

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

-----Original Message-----
From: Corbin, Clay via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, 5 October 2021 7:58 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Fixative in diff-quick

Hey folks,
I am shopping for a diff-quick kit.  However, all I really need is the fixative.  Generally, there is a blue stain (triarylmethane) added to the methanol in the fixative solution.  I have a giant jug of lab grade methanol.  What would I lose by using methanol alone compared to the fixative solution included in a diff-quick kit?

Clay Corbin, PhD
Professor of Biology
Bloomsburg University
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Histonet at lists.utsouthwestern.edu

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